Special Strains
Bielschowsky’s Silver Stain kit
Axons, neurofibrillary tangles and senile plaques: black
Background : yellow to brown
Verhoeff-Van Gieson (VVG) elastic stain kit
Elastic fibers: Blue-black to black
Nuclei: Blue to black
Collagen: Red
Other tissue elements: Yellow
AFB (ACID FAST BACILLI) kit
Acid fast bacilli: Red.
Nuclei: Blue.
Other tissue constituents: Blue.
ABPAS (ALCIAN BLUE AND PAS) kit
Acidic Mucins: Blue.
Neutral Mucins: Magenta.
Mixtures of above: Blue/purple
Nuclei: Deep blue.
Prussian Blue Staining for Iron kit
Iron (ferric form): Bright Blue
Nuclei: Red
Cytoplasm: Pink
Alcian Blue Staining kit
Strongly acidic sulfated mucosubstances: Blue
Nuclei: Pink to Red
Cytoplasm: Pale pink
MYELOPEROXIDASE (MPO) STAIN kit
Positive MPO Reaction
-
Neutrophilic granulocytes except blast forms
-
Eosinophils
-
Monocytes except blast forms
Negative MPO Reaction
-
Basophils (weakly positive)
-
Lymphocytic cell series
-
Erythrocyte cell series
Hematoxylin and Eosin (H&E) kit
Nuclei: Blue
Collagen: Pale pink
Muscle: Deep Pink
Acidophilic: cytoplasm Red
Basophilic: cytoplasm Purple
Erythrocytes: Cherry red
MODIFIED HIGH pH CONGO RED kit
Amyloid, Elastic Tissue, and Eosinophil Granules: Red
Nuclei: Blue
Giemsa’s Stain kit
Erythrocytes: Light pink to brown
Lymphocytes: Blue with purple nucleus
Eosinophil: Blue violet
Eosinophilic Granules: Deep red
Neutrophil: Deep blue to blue-violet
Neutrophil granules: Red
Basophil: Blue violet