IHC | Bioartis Life Scienc

Immunohistochemistry (IHC)

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AFB

Acid Fast for visualizing:

· Mycobacterium species

· Cryptosporidiosis Detection

· Detects oocytes in cryptosporidiosis.

Staining Results:

· Mycobacterium stain red,

· nuclei and other tissue elements stain blue.

· Cryptosporidiosis, cysts appear as reddish masses.

Massions Trichrome

Masson's Trichrome stain

· Differentiate &visualize various types of connective tissues

Staining Results:

· Collagen stains Blue

· Nuclei stains Black

· Muscle, cytoplasm & keratin stains Red

ALCIAN BLUE

Alcian Blue for visualizing

· Acid mucosubstances, Acidic mucins (lower PH).

· Familiar to identify: Mesothelioma, atherosclerosis and

adenocarcinomas.

Staining Results:

· Nuclei Stains pink – red,

· Cytoplasm stains pink

· Sulfated Acidic Mucosubstances stains Blue.

PAS ALCIAN BLUE STAIN

Pas Alcian Blue Stain

• Used for colon tissues that leads to Colorectal Carcinoma

• Distinguishes neutral mucins from acidic mucins .

Staining Results:

• Acidic mucins stains Blue

• Neutral mucins stains Magenta .

• Nuclei stains Deep blue.

Modified high Ph Congo red

Modified pH Congo red (Thioflavin T (CR-TT) stain) for visualizing

Amyloid Deposits: Atypical proteinaceous deposits in organs like liver, kidneys, spleen, etc.
Staining Results:
Amyloid, Elastic Tissue, Eosinophil Granules: Red;
·Nuclei: Blue.

Alcian Blue Hematoxylin/ Orange G Stain

• Discriminates the Mature bone, Immature bone & cartilages in Osteoarthritis (OA)

Staining Results:

• Activated osteocytes stains Bright blue

• Cartilage stains blue/Purple

• Bone stains orange-red.

Alizarin Red

calcium deposits in a tissue
Characterizes the enlargement of bone Osteoporosis.
Calcium deposits stains Orange – Red.

Gram Stain

Visualize bacterial infection in throat, lungs, genitals, or in skin wounds.
As gram-positive and gram-negative bacteria have different susceptibilities to different antibiotics.

Bielschowskys Silver Stain

It imparts both the amyloid and neurotic components.
Detection of Alzheimer's diseases

Gomori’s Blue Trichome

Used in musculoskeletal &skin disorders that affects connective tissue (scleroderma).
Increase in collagen fibers in the connective tissue or to differentiate collagen and smooth muscle fibers.

Giemsa Stain:

Detect parasites such as malaria and Treponema palladium in blood smears, and gastrointestinal biopsies.
The Giemsa stain was unique due its staining of chromatin, nuclear membranes, and cytoplasmic elements.

Gomoris Green Trichome

Detects the increase in collagen fibres in tissues and identifies muscle fibres, nuclei.
Used in the diagnosis of various Musculoskeletal Disorders
Muscle fibers stains Red, Collagen fibers stains Green, nuclei stainsBlue.

Grocott’s Methenamine Silver

The identification of fungi on cytosmears and tissue sections.
It imparts black to fungal specimens and background pale green (counter stain).

Malachite Green Stain

Detects Cryptosporidium oocytes in fecal and environmental samples.
It imparts Cryptosporidia as bright red and background blue green.

Oil Red O Stain

To visualize neutral lipids, in adipocytes or fat cells.

Pas (Periodic And Schiff) Stain

Detect glycogen deposits in liver
Glycogen, Mucin and Some Basement membranes imparts Red/Purple& counterstains appears green / blue.

Pas Diastase Stain:

PAS stain in combination with diastase, which is an enzyme that digests the glycogen.
It promotes digestion in cases of chronic illness, stomach fullness, flatulence, and indigestion.
Glycogen is digested not stained other cells imparts magneta

.

Prussian Blue Stain

Used in the detection of ferric iron in tissues,
Iron (ferric form) imparts Bright Blue, nuclei imparts red, and cytoplasm imparts pink...

Verhoeff’s Elastic Stain

Detects abnormal patterns of elastic fibers as found in blood vessel walls, elastic cartilage, lungs, skin, bladder, and some ligaments.
Elastic fibres imparts Blue-Black over yellow background.

Von Kossa Stain

Detects adequate calcium deposits in different tissues (joints, blood vessels and kidneys are most often affected).
Tissues are impregnated with silver to reduce calcium.
Calcium imparts black, nuclei and cytoplasm appears red, pink.

Mucicarmine Stain

Detection of mucins of epithelial origin,including tumors, cysts, and infections.
Useful in detecting adenocarcinomas originating from the gastrointestinal tract.
Mucins appears as Magenta whereas Nucleiappears as blue.